pepT [2019-08-12 13:34:48]

peptidase T (tripeptidase), zinc-dependent

locus: BSU_38920

pI: 4.54

mw: 45.35 kDa

protein length: 410 aa

gene length: 1233 bp

function: peptide degradation

product: peptidase T (tripeptidase)

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.4|Utilization of peptides]

Gene

Coordinates: 3,995,075 → 3,996,307

The protein

Catalyzed reaction/ biological activity

• Release of the N-terminal residue from a tripeptide (according to Swiss-Prot)

Protein family

• peptidase M20B family (single member, according to UniProt)

Paralogous protein(s)

• [protein|4D56C583BBAF47F384ACFACF7A607FD8BFA59960|YqjE]

Structure

• [PDB|3GBO] (from ''Bacillus cereus (71% identity''), to be published)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|B77F24856B19A8101EDDB8A95D4E201F44C789DC|pepT]
  description: [Pubmed|10746760]

Biological materials

Mutant

• MGNA-B733 (pepT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1732 NBRP B. subtilis, Japan]
• BKE38920 (Δ[gene|B77F24856B19A8101EDDB8A95D4E201F44C789DC|pepT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCAATCATCTCCTTT, downstream forward: _UP4_TAACGCCAAAAGCCAGTCCA
• BKK38920 (Δ[gene|B77F24856B19A8101EDDB8A95D4E201F44C789DC|pepT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCAATCATCTCCTTT, downstream forward: _UP4_TAACGCCAAAAGCCAGTCCA

References

• 8978088,10987140